Coding

Part:BBa_K2764000:Design

Designed by: CHU Ora-In   Group: iGEM18_Hong_Kong_HKUST   (2018-10-03)


Alkane channel protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 651
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 303
    Illegal NgoMIV site found at 516
  • 1000
    COMPATIBLE WITH RFC[1000]

The part sequence has been codon-optimized for E. coli.

Design Notes

Added promoter and terminator for expression


Source

outer membrane protein AlkL from Pseudomonas oleovorans

References